To investigate the methodologies and focal points of standardized clinical training programs for brain death diagnosis in secondary general hospitals, ensuring the quality of such training. Methods: The Second Affiliated Hospital of Hainan Medical University served as a pilot site for this standardized training initiative, offering a four-phase course to 79 trainees from 33 secondary general hospitals spanning 17 counties and cities within the province. The training comprised three stages: pre-training baseline assessment, establishment of training goals and adaptation of key training elements, and post-training feedback collection. An analysis and summary of the training protocol, achievements, and satisfaction levels were conducted. Results: Post-training, all 79 trainees achieved the qualifying criteria for brain death diagnosis training (with a correct response rate exceeding 70%), yielding a 100% pass rate. Satisfaction rates with the training schedule and format stood at 88.7%, while the content and delivery of instruction received satisfaction ratings of 93.6% and 91.9% respectively; the overall evaluation of the training garnered a satisfaction rate of 93.6%. With ongoing refinement and optimization of the training scheme, there was a statistically significant incremental improvement in assessment scores across the phases (F=8.89, P<0.01). Pairwise comparisons revealed that scores in phases 2, 3, and 4 were significantly higher than in phase 1 (P<0.05), with phase 4 also surpassing phase 2 (P<0.05). Con? clusion: The pilot training program of Hainan Province for standardized clinical determination of brain death in secondary general hospitals exhibited superior training quality, thereby securing the proficient execution of brain death determinations. This model can inform the broad implementation of standardized clinical training for brain death diagnosis in secondary general hospitals across the nation.