To investigate the correlation between tumor-associated fibroblasts (TAF) in pituitary tumor and tumor size, aggressiveness, texture, and postoperative pituitary function. Methods: The clinical data of 112 pituitary adenoma patients treated with pituitary adenoma by transnasal sphenoidal approach surgery was retrospectively analyzed. The pituitary adenoma tissues of 24 cases were collected and immunohistochemical staining was performed to detect three biomarkers of TAF: α-SMA, PDGFRB and TAGLN. Patients were divided into hypopituitarism group and normal function group according to the postoperative hormone test results. And patients were divided into hard texture group and soft texture group according to texture of tumors. The correlation between TAF in pituitary tumor and tumor size, aggressiveness, texture, and postoperative pituitary function were analyzed. Results: (1) Compared with the normal function group, the hypopituitarism group had higher proportion of men, older age, larger tumor size, higher Knosp grading and higher proportion of hard texture (all P<0.05). (2) Compared with the normal function group, the hypopituitarism group had higher expressions of α-SMA, PDGFRB and TAGLN (all P<0.01). Compared with the soft texture group, the hard texture group had higher expressions of α-SMA, PDGFRB and TAGLN (all P<0.01). And expression level of TAF was significantly positively correlated with the size of pituitary tumors and the Knosp grading (all P<0.01). Conclusion: TAF in pituitary tumor are expressed in higher amounts in patients with hypopituitarism after surgery and in patients with harder tumor texture. The level of TAF in pituitary tumors is significantly positively correlated with the size and aggressiveness of pituitary tumors.