To investigate the differential expression of circRNA and its function in hippocampal tissues of epileptic rats induced by lithium chloride-pilocarpine. Methods: The epileptic rat model was established using lithium chloride-pilocarpine. Hippocampal tissue of epileptic rats and control rats was collected for transcriptome sequencing by the Illumina high-throughput sequencing platform. The differences in circRNA were analyzed with the edgeR package. GO and KEGG pathway enrichment analysis was performed in R. Targetscan and miRanda were used for circRNA-miRNA interaction software analysis to find circRNA core control targets. Results: We obtained 262 differentially expressed circRNAs by differential analysis of hippocampal circRNA in the control group and epilepsy group. Signal pathway enrichment analysis revealed that these circRNAs were associated with the cytoprotein modification process, protein degeneration process, dendritic morphology regulation, neuron differentiation regulation, ion transmembrane transport, and other biological processes. We further identified core regulatory miRNAs that may interact with circRNA, including miR-322-5p, miR-322-3p, miR-323-5p, miR-323-3p, miR-301a-5p, miR-301a-3p, miR-324-5p, miR-324-3p, etc. Conclusion: In this study, the difference in brain tissue circRNA between lithium chloride-pilocarpine epileptic rats and normal rats was screened and the possible mechanism of function was systematically analyzed so as to find a reliable biomarker for the diagnosis and treatment of epilepsy.