To explore effectiveness of the regional collaboration stroke quality control platform in the emergency green channel. Methods: Acute ischemic stroke (AIS) patients who visited the emergency green channel in the Department of Neurology of Wuhan First Hospital from May 2017 to April 2018 were enrolled as control group, and AIS patients who visited the regional collaboration stroke quality control platform in the same department from May 2018 to April 2019 were enrolled as study group. The thrombolysis rate, door-to-needle time (DNT), subsequent visit rate 3 months after onset, and change in modified Rankin Scale (mRS) score 3 months after onset were studied in the two groups. Results: The study group showed improved thrombolysis rate, DNT, subsequent visit rate, and mRS score compared to the control group (all P<0.05). Conclusion: The regional collaboration stroke quality control platform can optimize the emergency treatment process in each stroke center and reduce the overall emergency treatment time.