To perform a quantitative study to determine the degree of sciatic nerve injury in SD rats caused by varying clamp forces and established a new model of sciatic nerve injury in accordance with the Sunderland Ⅴ classification. Methods: A force measurement was performed on JZ06Cr 14 cm hemostatic forceps. JZ06Cr 14 cm hemostatic forceps were used in rat model generation. Thirty-two 4-week-old healthy clean-grade male SD rats were randomly divided into groups control (n=8) and pressure [n=32, including hemostatic forceps first ratchet position subgroup (8 rats), second ratchet position subgroup (8 rats), and third ratchet position subgroup (8 rats)]. All groups were measured for nerve conduction velocity (NCV) 30 minutes after modeling, and the nerves were obtained for HE staining and observation. Results: Force measurements of JZ06cr 14 cm hemostatic forceps showed that the force generated by the first ratchet position was approximately 16 N, that of the second ratchet position was approximately 31 N, and that of the third ratchet position was approximately 46 N. Sciatic nerve NCV of the first ratchet position group showed significant difference compared to that of the control group (P<0.05). Examination of morphology showed nerve fibers arranged in parallel; dense structure; and intact axons, myelin sheaths, and endomembrane without rupture. Sciatic nerve NCV of the second ratchet position group showed significant difference when compared to that of the control group and first ratchet position group (P<0.05). Morphologically, the nerve fibers were broken, the endomembrane partially damaged, and the structure disordered. In the third ratchet position group, the NCV of some nerves could not be measured, and there was statistical significance compared to all other groups (P<0.05). Observation of morphology revealed broken nerve fibers and intact epineurium and perineurium. Conclusion: Approximately 16 N of clamp force creates the sciatic nerve clamp injury model in accordance with Sunderland Ⅰ degree injury; approximately 31 N accords with Sunderland Ⅱ or Ⅲ degree injury, and approximately 46 N accords with Sunderland Ⅲ degree injury.