To investigate whether there is an interaction between HIF-1α and Notch1 in co-cultured neurons and glial cells. Methods: Cortical neurons and glial cells were co-cultured in vitro. The HIF-1α and Notch1 genes in the co-cultured cells were muted by electroporation transfection, then the transcription levels and protein expression levels of Notch1 and HIF-1α were detected by RT-PCR and Western Blot, respectively. Results: After silencing either the HIF-1α or Notch1 gene, the transcription and protein expression levels of both HIF-1α and Notch1 were reduced. Conclusion: There may be an interaction between HIF-1α and Notch1 in the neuron and glial cell co-culture system