To study the effect of selective optogenetic regulation by Channelrhodopsin2 (ChR2) on intracellular calcium levels in astrocytes. Methods: Astrocytes were cultured from SD neonate rats, and a lentiviral vector, Lenti-GFAP-ChR2-YFP, was used to transduce cultured astrocytes. Astrocytes were stimulated using blue light with a wavelength of 470 nm, and intracellular calcium signals, as indicated by the fluorescent Ca2+ indicator Rhod-2 AM, were recorded in real time by confocal fluorescence microscopy. Results: ChR2 was successfully expressed in astrocytes. Photostimulation of ChR2 in astrocytes led to the activation of intracellular calcium signaling and an increase in calcium levels. Conclusion: The use of ChR2 as an essential element of optogenetics could be a powerful tool in the regulation of calcium in astrocytes.