To study the effect of unactivated/activated human microglia cell line CHME-5 on the migration and invasion of nasopharyngeal carcinoma cell line CNE-2. Methods: Human microglia cell line CHME-5 was cultured, and 1 μg/mL LPS was used to activate cells. Unactivated/activated cell supernatant was collected. For the experiment, we established 6 groups: control group, 45% supernatant group, 90% supernatant group, LPS group (LPS activated microglia supernatant), 45% LPS supernatant group, and 90% LPS supernatant group. The wound healing assay and Transwell inserts were used to establish the nasopharyngeal carcinoma cell line CNE-2 migration model, and the effect of unactivated/activated microglia supernatant on the migration of the cell was observed. Transwell inserts were used to establish the nasopharyngeal carcinoma cell line CNE-2 invasion model, and crystal violet staining was used to observe the effect of microglia supernatant on the migration of the cell. Results: Low (45%) and high (90%) concentrations of unactivated/activated microglia supernatant promoted the migration and invasion of nasopharyngeal cancer cells. Conclusion: Both unactivated and activated microglia supernatant promote the migration and invasion of nasopharyngeal carcinoma cells.