To investigate the relationship between coagulation and the changes in levels of lnterleukin-6 (IL-6), IL-8, and tumor necrosis factor-α (TNF-α) in patients with acute cerebral infarction. Methods: We recruited 74 patients with acute cerebral infarction. Patients were divided into the mild stroke group (n=41) and moderate-to-severe stroke group (n=33) according to severity of symptoms based on the National Institute of Health stroke scale (NIHSS) score. In addition, 30 patients without cerebral infarction and hospitalized during the same time as the cerebral infarction group were recruited as the control group. Coagulation was evaluated by thromboelastography, and serum levels of IL-6, IL-8, and TNF-α were detected by chemiluminescence analysis. We studied changes in cytokines between the groups and analyzed their relationship with coagulation. Results: The G-value reflecting clot hardness was higher in the cerebral infarction group than in the control group (P<0.05), and the increase in G-value and MA was greater in the moderate-to-severe stroke group compared to the control group (P<0.05). Compared to the IL-6 and TNF-α levels of the control group, those of the cerebral infarction group were higher (P<0.05) and those of the moderate-to-severe stroke group were significantly higher (P<0.05). IL-8 level in the moderate-to-severe stroke group was higher than that in the mild stroke group (P<0.05). Changes in serum TNF-α levels in cerebral infarction patients showed a positive correlation to G-value in TEG (r=0.295, P=0.015). Conclusion: IL-6 and TNF-α are involved in the coagulation of blood in patients with cerebral infarction. IL-8 is related to the severity of disease. TNF-α is related to the hardness of the clot which may be an important factor in promoting thrombosis in patients with cerebral infarction.