To investigate the effect of Adam10 gene on the cross-talk between astrocyte and pericyte, and on the integrity of blood-brain barrier (BBB). Methods: Adam10 gene knockout mice (Gfap cre -Adam10 lox/lox ) were obtained by Cre-loxp technique. Adam10 lox/lox mice were used as control. The expression of myocardin and smooth muscular actin (SMA) protein in pericytes was detected by double immunofluorescence labeling technique. The degeneration and apoptosis of pericytes, destruction of basement membrane and BBB were analyzed by electron microscopy. Laser speckle imaging was used to observe the changes of regional cerebral blood perfusion. Results: Specific knockout of Adam10 gene in astrocytes affectted the signal exchange between astrocyte and pericyte; decreased the myocardin and SMA protein expression in pericytes; induced pericyte degeneration and apoptosis; damaged BBB; and affected vascular density and blood flow velocity in brain cortex. Conclu? sion: Adam10 gene may affect the signal exchange between astrocytes and pericytes, thus affecting the integrity of BBB and cerebral blood perfusion.