To analyze the distribution of the SLCO1B1 gene 388A＞G and 521T＞C polymorphic loci in 185 patients with large artery atherosclerosis-subtype ischemic stroke (LAA-IS). Methods: This study recruited 185 LAA-IS patients as research subjects. The 388G>A and 521T>C loci of the SLCO1B1 gene in the peripheral blood samples were detected by the polymerase chain reaction fluorescence probe method, and the distribution of their genotyping was statistically analyzed. Results: According to the polymorphism of the SLCO1B1 gene in the 185 LAA-IS patients, at the 388A>G site, homozygous wild type accounted for 8.6%, heterozygous mutant type accounted for 40.5%, and homozygous mutant type accounted for 50.8%; at the 521T> C site, homozygous wild type accounted for 77.8% , heterozygous mutant type accounted for 21.0% , and homozygous mutant type accounted for 1.1%. Conclusion: Within the 185 cases in this study, there exists a large distribution of mutant genotypes of the SLCO1B1 gene.