To systematically evaluate the clinical efficacy of non-invasive neuromodulation techniques combined with virtual reality (NINT-VR) for upper limbs motor dysfunction in stroke patients. Methods: A comprehensive search in databases of PubMed, Embase, Web of Science, Cochrane Library, Medline, CNKI, Wanfang, VIP Database was performed for randomized controlled trials (RCTs) of NINT-VR for upper limbs motor dysfunction in stroke patients. The search time was from databases inception to September 2024. Quality assessment was performed using Physiotherapy Evidence Database (PEDro). Meta-analysis was performed using RevMan 5.4.1. Network meta-analysis was performed and funnel plots were drawn to test for publication bias of the outcome indicators using Stata 17.0 software. Results: A total of 12 RCTs involving 538 patients were included. The meta-analysis results showed that, NINT-VR improved fugl-meyer assessment upper extremities scale (FMA-UE) (MD=8.32, 95% CI 6.18～10.45, P<0.00001) in stroke patients. Action research arm test (ARAT) score (MD=9.58, 95% CI 6.24～12.91, P<0.00001) and modified barthel index (MBI) score (MD=11.08, 95% CI 8.52～13.65, P<0.00001) also had been improved compared to the control group. Subgroup analysis showed that NINT-VR intervention plan and duration of single treatment were significant sources of heterogeneity. FMA-UE score could be improved by NINT-VR for both less than 30 minutes at a time (MD=4.68, 95%CI 1.93～7.43, P=0.0008) and more than 30 minutes at a time (MD=10.22, 95% CI 8.55～11.89, P<0.00001). For the effect of NINT-VR intervention plan, the cumulative probability ranking of network meta-analysis were transcranial direct current stimulation (tDCS) and VR (non-simultaneous)>transcranial magnetic stimulation (TMS) and VR (non-simultaneous)>tDCS and VR (simultaneous) >VR treatment. Conclusion: NINT-VR demonstrates significant benefits for improving upper limbs motor dysfunction and activity of daily living (ADL) in stroke patients, and tDCS and VR (non-simultaneous) was one of the most effective intervention plan.