To investigate the role of LncRNA MIRLET7BHG in human neuroblastoma cell line
(SH-SY5Y) during ischemia reperfusion injury. Methods: An oxygen and glucose deprivation/reoxygenation
(OGD/R) model was used to simulate in vitro ischemia reperfusion injury in SH-SY5Y cells, and the level of
LncRNA MIRLET7BHG was examined by RT-qPCR. A LncRNA MIRLET7BHG knockdown model of
SH-SY5Y cells was constructed, and MTT, flow cytometry, and Western blotting were used to evaluate the
viability and apoptosis levels of cells after normal culture or OGD/R treatment. Results: After OGD/R
treatment, the apoptosis rate of SH-SY5Y cells increased significantly, the cell survival rate decreased
significantly, the levels of oxidative stress markers increased significantly, and the transcription level of LncRNA
MIRLET7BHG increased significantly. Knocking down LncRNA MIRLET7BHG in SH-SY5Y cells reduced the
viability of SH-SY5Y cells under normal culture or OGD/R treatment and promoted cell apoptosis.
Conclusion: LncRNA MIRLET7BHG has a certain inhibitory effect on OGD/R-induced apoptosis of
SH-SY5Y cells. The up-regulation of LncRNA MIRLET7BHG transcription level suggests that there may be a
negative feedback mechanism.
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