To explore mediating role of health behaviors between disability acceptance and quality of life in young and middle-aged stroke patients. Methods: From July 2019 to February 2022, 192 cases of young and middle-aged stroke inpatients from the Department of Neurology of a tertiary care hospital in Wuhan were selected. Questionnaires were conducted using the general information questionnaire, Acceptance of Disability Scale-Revised (ADS-R), Health Promoting Lifestyle Profile Ⅱ (HPLP Ⅱ), and World Health Organization Quality of Life-BREF (WHOQOL-BREF). Results: The ADS-R score was (81.13 ± 10.58), the HPLP Ⅱ score was (124.47 ± 25.17), and the WHOQOL-BREF percentile score was (59.59 ± 10.52). Correlation analysis showed that both disability acceptance and health-promoting lifestyles were positively associated with quality of life (P<0.01). The mediating effect of health behaviors between disability acceptance and quality of life accounted for 13.74% of the total effect. Conclusion: Disability acceptance, health-promoting lifestyle, and quality of life are all moderate in young and middle-aged stroke patients. Health-promoting behavior plays a partially mediating role between disability acceptance and quality of life.