This study aimed to investigate the effects of immersive virtual reality (VR)-based walking rehabilitation overground training with walkers on the postural balance and walking ability of stroke patients. Methods: A total of 40 patients with ischemic stroke who could independently walk a minimum of 10 m were included in this study. The participants were randomly divided into two groups: a control group comprising 21 subjects and an observation group comprising 19 subjects. The patients completed either conventional walking rehabilitation training or immersive VR-based walking rehabilitation overground training using walkers combined with conventional training. At the baseline and four weeks after treatment, patients’walking abilities and daily activity performance were assessed by using the Tinetti and balance gait scale (TGS), 10-m walking test, and the Barthel index (BI). The static balance and walking balance data and gait data were collected using a balance detector and smart sensing walker, respectively. Results: The measurement results of the balance detector showed improved static balance levels in both the observation and control groups compared to pre-training levels. After rehabilitation, the horizontal movement amplitude and coverage area of the center of gravity of the observation group were significantly reduced compared with those of the control group (P<0.05) when the eyes were open and the arms were akimbo. Additionally, the total movement and vertical movement rates of the center of gravity in the observation group were significantly reduced compared with those of the control group when the eyes were open and the arms were extended forward (P<0.05). After training, the TGS scores, walking speed, and BI were improved in both groups, and no significant differences were observed between the two groups. However, analysis of the patients’gait data recorded using the smart sensing walker revealed better improvements in the control of the center of gravity in the observation group than in the control group (P<0.05). Conclusion: Immersive VR-based walking rehabilitation overground training with walkers improved the walking ability of stroke patients, particularly balance control, meriting further research and application.