To explore the application of AI-assisted compressed sensing (ACS) technology combined with animation audio playback in head magnetic resonance imaging (MRI) examination of preschool children. Methods: A total of 160 preschool children undergoing head MRI examination were selected as the study subjects, including 80 cases in the study group and 80 cases in the control group. The study group underwent MRI sequence scanning with ACS technology and animated audio was played during the examination, while the control group was given oral chloral hydrate before the examination and underwent conventional head MRI sequence scanning. The image quality, examination success rate, and patient satisfaction of the two groups were compared and analyzed. Results: The examination success rate of the study group was 97.5%, which was higher than that of the control group (77.5% ) (P<0.05). The examination time of the study group was (104.44±2.98) s, which was significantly shorter than that of the control group (342.86±13.36) s (P<0.05). The family satisfaction rate of the study group was 100%, which was higher than that of the control group (80% ) (P<0.05). There was no significant difference in signal-to-noise ratio and subjective image quality between the study group and the control group (P>0.05). Conclusion: The playback of animation audio can effectively help preschool children complete MRI examination without using sedatives, while ACS technology can significantly shorten the examination time of MRI. The combination of the two greatly improves the success rate of head MRI examination in preschool children, which is worth promoting.