To explore optimal conditions for the suture occlusion method to make the model of hemorrhage transformation after cerebral ischemia in rats. Methods: The rat model of transient middle cerebral artery occlusion (tMCAO) was established by the suture occlusion method. According to the changes in cerebral blood flow and results of neurological assessment 2 hours after operation, the rats that underwent successful model establishment were selected and randomly divided into 3 groups. The suture was removed at 3, 6, and 9 hours after cerebral ischemia to make hemorrhage transformation after cerebral ischemia (tMCAO-hemorrhagic transformation, tMCAO-HT). The cerebral infarction, incidence of HT, cerebral hemorrhage volume, hemoglobin content of brain tissue and mortality rate were used as indicators at 48 hours postoperatively to screen for optimal tMCAO-HT rat model replication conditions. Results: Compared with the sham operation group, the cerebral blood flow in each model group measured 5 minutes after cerebral ischemia decreased to 42%～53% of the baseline value; when measured 5 minutes after reperfusion, the blood flow in each model group was significantly increased. The neurological scores at 2 hours after operation were significantly increased (P<0.01), and there was no significant statistical difference between the groups. There was obvious cerebral infarction and HT in various degrees in each model group 48 hours after operation. The HT hemorrhage volume and hemoglobin content in each model group were significantly greater than those of the sham operation group (P<0.05 or P<0.01), and these values gradually increased with the prolongation of ischemia. Each model group showed different degrees of mortality, and mortality rates increased with the increased ischemia time, in which the mortality rate of the 9-hour ischemia group reached 83.33%. Conclusion: The tMCAO-HT model in rats can be successfully replicated by reperfusion at 3, 6, and 9 hours after cerebral ischemia with the suture method. However, the mortality rate at 9 hours is too high, and reperfusion after 3 or 6 hours of cerebral ischemia is more appropriate in studies using the tMCAO-HT model.