To investigate clinical features of neurobrucellosis (NB). Methods: The clinical data of 3 NB patients were retrospectively analyzed and combined with relevant literature to further analyze clinical feature of the disease. Results: The 3 NB cases were all male patients with a definite epidemiological history of contacting with cattle and sheep. Case 1 presented with fever and hearing loss upon onset then gradual limb weakness. Case 2 displayed progressive weakness of both lower limbs and lower back pain at the late stage of disease. Case 3 presented with stroke at onset then gradually developed joint pain and spinal cord damage. Routine cerebrospinal fluid (CSF) examination of all 3 cases showed a mild increase of white blood cells (WBCs), increase of protein, and decrease of glucose and chloride. The CSF culture was positive for brucella in 1 case, and all 3 cases were positive for the brucella agglutination test. Two patients displayed abnormal signals on MRI. Two patients improved after regular treatment, and 1 patient relapsed. Data of 310 NB cases were collected from MEDLINE. Peripheral nerve involvement was frequently reported, and chronic meningitis was the most common impairment of the central nervous system. Conclusion: NB is a rare disease. Patients living in endemic areas, who contacted cattle or sheep, and who present with undulant fever and related impaired neurological signs should be considered for NB. Etiological examination should be performed as soon as possible. Early treatment with antibiotics is effective and yields a favorable prognosis.