To compare the different effects of inducing human pluripotent stem cells to differentiate into medial ganglionic eminence (MGE) neural progenitor cells with monolayer adherent culture and embryoid bodies (EB) culture methods and to explore the effect of different concentrations of SAG (SHH signaling agonist) on the purity of neural progenitor cells differentiated into MGE. Methods: Human pluripotent stem cells were differentiated into neuro epithelial cells using monolayer adherent culture and EB culture methods. Varying concentrations of SAG were used to induce ventralization. The cells were ultimately induced to become NKX2.1-expressing MGE neuro progenitor cells and then counted. The effect of the two culture methods and the different concentrations of SAG on the purity of MGE neural progenitor cells were investigated. Results: In the monolayer adherent culture method, the positive expression of NKX2.1 was 0% in 0 μM SAG, (48.3±11.5)% in 0.1 μM SAG, (78.6± 2.7)% in 0.5 μM SAG, (77.6±4.0)% in 1 μM SAG, (69.0±7.5)% in 2 μM SAG, and (67.7±7.9)% in 3 μM SAG. The purity of differentiated MGE neural progenitor cells induced by 0.5μM and 1μM SAG was superior to those induced by other concentrations (P<0.001 or 0.05). Using the EB culture method with 0.5 μM SAG, the positive expression of NKX2.1 was (74.8±6.5)% and showed no significant difference from the monolayer adherent culture method (P>0.05). Neurosphere and neuron formation occurred earlier when using the monolayer adherent culture method than when using the EB culture method. Conclusion: To obtain MGE neural progenitor cells from human pluripotent stem cells for transplantation treatment, the monolayer adherent culture method with 0.5 μM SAG may be optimal.