To investigate the efficacy of surface electromyographic biofeedback (sEMGBF) therapy combined with nerve mobilization (NM) on hand function improvement in recovery-stage stroke patients. Methods: Total 84 recovery-stage stroke patients were enrolled and were randomly divided into groups control and treatment with 42 patients in each group. All patients received routine upper extremities function therapy, and treatment group received additional sEMGBF therapy combined with NM. Treatments were performed once per day; sEMGBF was used 20 min per treatment and NM applied 15 min per treatment. Therapies in both groups were given 5 days per week for 6 consecutive weeks. The upper extremities Fugl-Meyer (U-FMA) score, active range of motion (AROM), muscle strength of wrist stretching (MMT), and modified Bathel Index (MBI) before and after treatment were evaluated. Results: Compared to before treatment, improvements in U-FMA, AROM, wrist stretch muscle strength, and MBI was observed in both groups after 6 weeks of treatment, and results were statistically significant (P<0.05); in addition, improvements in the treatment group were greater than in the control group (P<0.05). Conclusion: sEMGBF combined with NM strategy can improve the hand function of recovery-stage stroke patients leading to improvement in upper extremities function and improved self-care in activities of daily living.