A new experimental device for diffuse axonal injury (DAI) was designed and manufactured in which the basic mechanism was the combination of angular and linear acceleration. The device was able to replicate graded DAI of different severity by regulating the mechanical parameters. Methods： Thirty-six mice were randomly divided into the control group (n=6) and injury group (n=30); the injury group was further divided into the mild, moderate, and severe subgroups with 10 mice in each. The mice in the three injury subgroups were subjected to coronal rotation after weight-drop impact injury using a newly manufactured device; the mechanical parameters (impact baton’s mass and rotation angle) of the mild, moderate, and severe DAI subgroups were 50g × 60cm/22.5° , 100g × 60cm/45° , and 150g × 60cm/90° , respectively. The mice were sacrificed 24 hours post-injury, and immunohistochemical visualization of amyloid precursor protein (APP) and neurofilament (NF-200) was performed to assess characteristics of axonal damage. Behavioral changes of the injured mice were also evaluated. Results: All mice in the injury group showed longer latency for reflex recovery than mice in the control group, with the severe, moderate, and mild DAI subgroups respectively showing the longest, second longest, and shortest latency. The post-injury mortality rates of mice in the control group and the mild, moderate, and severe DAI subgroups were 0% , 0% , 10% , and 30% , respectively. Immunohistochemical visualization of APP and NF-200 showed varying degrees of positive staining; the severe DAI subgroup showed the strongest positive signal characterized by large amounts of nerve tissue swelling and axonal damage. The moderate DAI subgroup also displayed the above morphological characteristics but not with the same intensity as the severe DAI subgroup. Immunohistochemical staining of APP and NF-200 in the mild DAI subgroup showed only minimal, localized intracranial bleeding with relatively few positive signals. Conclusion: A novel device that combines linear and rotational acceleration injury mechanisms for establishing the DAI experimental model has been successfully created. Different degrees of injury can be induced by varying mechanical parameters to establish graded DAI in mice. This device demonstrates good usability, repeatability, and reliability.