To investigate the effect of plasma non-fasting triglyceride (TG) levels on the prognosis of patients with acute cerebral infarction. Methods: A total of 446 patients with acute cerebral infarction were retrospectively enrolled. The blood pressure, blood glucose, National Institutes of Health Stroke Scale (NIHSS) score, and plasma triglyceride (TG), total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), and high density lipoprotein-cholesterol (HDL-C) levels at time of admission were recorded. The prognosis of the patients was evaluated by modified Rankin Scale (mRS) at 3 months after onset, and univariate and multivariate Logistic regression were used to analyze the risk factors that influence prognosis. Results: Compared with the good prognosis group, the poor prognosis group had a higher age, BMI, NHISS score, fasting blood sugar, and prevalence of coronary heart disease (CHD) and atrial fibrillation (AF) and a lower level of non-fasting TG (P<0.05). Univariate regression analysis showed that age, BMI, history of smoking, CHD, AF, fasting blood glucose, NIHSS score, and plasma non-fasting TG were the prognostic factors of acute cerebral infarction (P<0.05). Multivariate Logistic regression analysis showed that a higher non-fasting TG level (OR=0.548, 95% CI 0.372~0.808, P=0.002) was a predictor of a good outcome in acute cerebral infarction. The area under the ROC curve of the non-fasting TG level was 0.669 (95%CI 0.618~0.702，P=0.000), and the sensitivity and specificity were 67.9% and 38.2% respectively when the non-fasting TG level was 1.455 mmol/L. Conclusion: Non-fasting TG is an independent predictor of a good outcome at 90 days after the onset of acute cerebral infarction.