目的：探究孕期运动对青春期雄性后代小鼠焦虑与抑郁样行为影响以及相关机制研究。方法：母鼠 怀孕第2天开始受人为控制进行跑轮运动（1 h/d），一直到生产日结束。分娩后每周评估母鼠的母性行为。 青春期雄性后代（产后4周）接受为期2周，每天3小时的慢性束缚应激后进行行为学测试，取海马组织检测 神经炎症和神经凋亡水平。结果：来自孕期运动增加母鼠母性行为，同时减少青春期后代雄鼠慢性应激后 焦虑与抑郁样行为，并抑制海马中的细胞凋亡和NLRP3相关的神经炎症激活。结论：母鼠在怀孕期间进行 跑轮运动后，哺乳期间对后代的母性行为增加，减少了青春期雄性后代慢性应激后焦虑和抑郁样行为，其 机制可能与抑制了海马神经元凋亡以及NLRP3炎症小体相关的神经炎症激活有关。

To investigate the effect of exercise during pregnancy on anxiety and depressive-like behaviors in adolescent male mice offspring and the underlying mechanisms. Methods: Pregnant female mice were subjected to controlled wheel running (1 h/d) starting from day 2 of pregnancy until the day of birth. Maternal behavior was assessed weekly after delivery. Adolescent male offspring (4 weeks postpartum) underwent 2 weeks of chronic restraint stress for 3 hours daily before behavioral testing, and hippocampal tissue was collected to measure levels of neuroinflammation and apoptosis. Results: Prenatal exercise increased maternal behavior in pregnant female mice, reduced anxiety and depressive-like behaviors in adolescent male offspring after chronic stress, and inhibited apoptosis and NLRP3-associated neuroinflammatory activation in the hippocampus. Conclusion: Wheel running during pregnancy increased maternal behavior in lactating female mice, reducing anxiety and depressive-like behaviors in adolescent male offspring after chronic stress, possibly by inhibiting hippocampal neuronal apoptosis and NLRP3 inflammasome-related neuroinflammatory activation.