星形胶质细胞对神经元突触的形成起着重要作用。但由于缺乏体内研究细胞之间互作蛋白的技术 手段，我们对星形胶质细胞与神经元的互作机制了解有限。近来，来自杜克大学的 Cagla Eroglu 团队和 Scott H. Soderling团队在Nature杂志上发表的文章，报道了一种利用在体化学遗传学的Split-TurboID方法， 并利用该方法鉴定了星形胶质细胞中与神经元表面互作的蛋白，发现星形胶质细胞NrCAM蛋白可通过与 神经元NrCAM互作，负调控星形胶质细胞的体积大小，并在神经元桥尾蛋白介导下促进抑制性突触形成。

Astrocyte plays a crucial role in neuronal synaptic formation. However, our understanding of astrocyte-neuron interaction is hindered by the lack of in vivo cell-cell interaction proteome technique. Recently, a paper published in Nature by Cagla Eroglu and Scott H. Soderling et al. reported a Split-TurboID method[1] , utilizing a in vivo chemico-genetic approach to identify the proteome between the astrocyte-neuron cleft. Among these proteins, they found the astrocytic NrCAM negatively regulates astrocyte territory size and neuropil infiltration volume via transmembrane homophilic interaction with neuronal NrCAM. In addition, this interaction promotes inhibitory synaptic formation with the participation of gephyrin.