目的：观察在原代混合培养的神经元及胶质细胞的体系中，HIF-1α和Notch1基因间是否存在相互作 用。方法：构建体外原代培养神经元及胶质细胞的混合体系，应用电穿孔转染方法分别沉默 HIF-1α和 Notch1基因。RT-PCR检测HIF-1α和Notch1基因转录水平，Western blot方法检测HIF-1α和Notch1蛋白表 达水平。结果：沉默HIF-1α基因后，HIF-1α和Notch1基因转录及蛋白表达水平均显著下降；沉默Notch1基 因后，HIF-1α和Notch1基因转录及蛋白表达水平均显著下降。结论：在原代培养的神经元及胶质细胞的混 合体系中，HIF-1α和Notch1之间可能存在相互作用的关系。

To investigate whether there is an interaction between HIF-1α and Notch1 in co-cultured neurons and glial cells. Methods: Cortical neurons and glial cells were co-cultured in vitro. The HIF-1α and Notch1 genes in the co-cultured cells were muted by electroporation transfection, then the transcription levels and protein expression levels of Notch1 and HIF-1α were detected by RT-PCR and Western Blot, respectively. Results: After silencing either the HIF-1α or Notch1 gene, the transcription and protein expression levels of both HIF-1α and Notch1 were reduced. Conclusion: There may be an interaction between HIF-1α and Notch1 in the neuron and glial cell co-culture system