目的：探讨Nrf-2/HO-1通路在糖尿病大鼠神经病理性痛中的作用。方法：SD 大鼠 24 只随机分为 3 组：对照组（C 组）、糖尿病组（D 组）、糖尿病+tBHQ 组（DT 组），每组 8 只。采用腹腔注射 1%链脲佐菌素 60 mg/kg 的方法制备糖尿病大鼠模型。DT 组于糖尿病模型制备成功后第 3 天，腹腔注射 tBHQ 30 mg/kg 至42 d。分别于第2，4，6周测定大鼠机械痛阈（MWT）及坐骨神经导速率（MNCV），6周后处死大鼠，Nissl 染色观察脊髓病理学变化，电镜观察腓肠神经病理学改变，Western blot法检测脊髓核转录因子红细胞系-2 相关因子-2（Nrf-2）、激活血红素氧合酶-1（HO-1）。结果：与C组比较，D组中脊髓Nissl染色和腓肠神经电镜 均显示神经损伤明显，MWT和MNCV明显降低，Nrf-2、HO-1表达降低（均P<0.05）；与D组比较，DT组中脊 髓Nissl染色和腓肠神经电镜显示神经损伤较轻，MWT和MNCV升高，Nrf-2、HO-1表达升高（均P<0.05）。 结论：Nrf-2/HO-1通路可能参与 了糖尿病神经病理性痛的调控。

To evaluate the role of the Nrf-2/HO-1 pathway in neuropathic pain in diabetic rats. Methods: Twenty-four male Sprague-Dawley rats were randomly divided into 3 groups (n=8 per group): normal group (group C), diabetes group (group D), and diabetes+tBHQ group (group DT). Diabetes was induced in rats by intraperitoneal injection of 1% streptozotocin at 60 mg/kg. After successfully establishing diabetes model, group DT was given tBHQ at 30 mg/kg from day 3 until day 42. Mechanical withdrawal threshold (MWT) and motor nerve conduction velocity (MNCV) were evaluated at 2, 4, and 6 weeks. After 6 weeks, rats were sacrificed and spinal cords were removed for determination of pathology change by Nissl staining; pathology change of the sural nerve was evaluated by electron microscopy (EM); Nrf-2 and HO-1 were detected by western blot. Results: Compared with group C, damage to spinal structure was significantly decreased, MWT and MNCV were significantly decreased, and Nrf-2 and HO-1 expression was down-regulated in group D (all P< 0.05). Compared with group D, spinal structure damage was minor, MWT and MNCV were increased, and Nrf-2 and HO-1 expression was up-regulated in group DT (all P<0.05). Conclusion: The Nrf-2/HO-1 pathway may be involved in the regulation of diabetic neuropathic pain.